Diagnosis of quinolone-resistantCoxiella burnetii strains by PCR-RFLP

Diagnosis of mutations by the PCR double RFLP method (PCR-dRFLP).

The diagnosis of mutations that are responsible for human genetic diseases is important, and several molecular biology techniques based on polymerase chain reaction (PCR) (1), have been specially developed for this purpose (2-8) . For some of these techniques it is the presence or the absence of a PCR product that constitutes the analytical step, ie proves the presence of the mutation. In order...

Molecular characterization of Leishmania infantum strains by kinetoplast DNA RFLP - PCR SHORT

Parole chiave Cane, Epidemiologia, kDNA RFLP-PCR, Leishmania infantum, Tipizzazione molecolare. Riassunto Il metodo più frequentemente utilizzato per la classificazione di Leishmania spp. è ancora rappresentato dalla caratterizzazione isoenzimatica sebbene sia un metodo laborioso e in alcuni casi non sufficientemente discriminante. Nel presente studio abbiamo utilizzato una reazione di amplific...

Diagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP

Background: Direct identification of Leishmania species in Giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. This study was designed to isolate Leishmani spp. from Giemsa-stained smears and to characterize them by PCR technique. Methods: A total of 48 Giemsa-Stained slides from confirmed cases of leishmaniasis were examined under a ligh...

PCR and RFLP Analysis for Identification and Typing of Helicobacter pylori Strains from Gastric Biopsy Specimens

COMPARISON OF 16S rRNA-PCR-RFLP, LipL32-PCR AND OmpL1-PCR METHODS IN THE DIAGNOSIS OF LEPTOSPIROSIS

Leptospirosis is still one of the most important health problems in developing countries located in humid tropical and subtropical regions. Human infections are generally caused by exposure to water, soil or food contaminated with the urine of infected wild and domestic animals such as rodents and dogs. The clinical course of leptospirosis is variable and may be difficult to distinguish from ma...